Bordetella pertussis on Bordet-Gengou agar. Colonies of B.pertussis (arrows) and Streptococcus oralis on B-G agar.
Colony morphology: small, shiny, round colonies, mercury-silver in color.

B. pertussis is identified by slow growth in primoculture (usually after 2 days), colony morphology, oxidase reaction, and slide agglutination with specific antisera.

Cultivation 72 hours, 36 °C in an aerobic atmosphere.

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