The CAMP test and reverse CAMP test on plates. The CAMP test with Streptococcus agalactiae. The reverse CAMP test with Corynebacterium pseudotuberculosis.

positive CAMP test, positive reverse CAMP test reaction

Another arrangement of the CAMP and the reverse CAMP test. Single straight streaks of the bacteria to be tested and a beta lysin-producing Staphylococcus aureus strain are made perpendicular to each other and about 3 mm apart on the surface of a sheep blood agar plate. After overnight incubation at 37 °C, a positive test is characterized by an arrowhead-shaped zone of complete hemolysis in the area into which both staphylococcal beta lysin and CAMP factor have diffused (CAMP test with S.agalactiae) or by inhibition of staphylococcal beta lysin (the reverse CAMP test with C.pseudotuberculosis).