Klebsiella pneumoniae bacteria

Klebsiella pneumoniae

Klebsiella pneumoniae picture klebsiella pneumoniae animated

Three different strains of Klebsiella pneumoniae on Endo agar with biochemical slope (see here). Klebsiella pneumoniae
is urea positive (blue color of the slope), metabolise glucose with production of gas (bubbles under a piece of glass -
in detail left down side of each plate) and is lactose positive (but on Endo agar its colonies often remain quite pale).
Unlike some similarly looking strains of Enterobacter cloacae is K. pneumoniae lysine "+", ornithine "-", arginine "-"
(E. cloacae lysine "-", ornithine "+", arginine "+").

Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose fermenting, facultative anaerobic, rod
shaped bacterium
found in the normal flora of the mouth, skin, and intestines. It is clinically the most important member
of the Klebsiella genus of Enterobacteriaceae. It naturally occurs in the soil and about 30% of strains can fix nitrogen in
anaerobic condition. As a general rule, Klebsiella infections tend to occur in people with a weakened immune system.
Many of these infections are obtained when a person is in the hospital for some other reason (a nosocomial infection).
The most common infection caused by Klebsiella bacteria outside the hospital is pneumonia.
New antibiotic resistant strains of K. pneumoniae are appearing, and it is increasingly found as a nosocomial
infection. Klebsiella ranks second to E. coli for urinary tract infections in older persons. It is also an opportunistic pathogen
for patients with chronic pulmonary disease, enteric pathogenicity, nasal mucosa atrophy, and rhinoscleroma. Feces are the most
significant source of patient infection, followed by contact with contaminated instruments.

Abbreviated from Wikipedia

Klebsiella pneumoniae basic characteristics

  • GRAM-NEGATIVE RODS
  • NON-MOTILE
  • NON-SPORE-FORMING
  • CATALASE: POSITIVE
  • OXIDASE: NEGATIVE
  • FACULTATIVELY ANAEROBIC

Tests for identification of Klebsiella pneumoniae

MacConkey
growth
Indole
production
Methyl
red
Voges-
Proskauer
Citrate
(Simmons)
Hydrogen
sulfide(TSI)
Urea
hydrol.
Lysine
decarb.
Arginine
dihydrol.
Ornithine
decarb.
POS. NEG. NEG. POS. POS. NEG. POS. POS. NEG. NEG.
Motility
(36 °C)
D-glucose
acid/gas
D-mannitol
ferm.
Sucrose
ferm.
Lactose
ferm.
D-sorbitol
ferm.
Cellobiose Esculin
hydrol.
Acetate
utiliz.
ONPG test
NEG. POS./POS. POS. POS. POS. POS. POS. POS. D POS.


  • POS. positive ( > 90% of strains are positive)
  • D most positive (51 - 89%)
  • d most negative (11 - 50%)
  • NEG. negative (0 - 10%)


  • Antibiotic treatment of Klebsiella pneumoniae infections

    Should be always guided by in vitro susceptibility tests!!
    Selection of appropriate antibiotics depends on diagnosis!!

    Uncomplicated urinary tract infections
    • trimethoprim-sulfamethoxazole
    • cephalosporines(second and third generation)
    • tetracyclines

    Alternative
    • aminoglycosides
    • amoxicillin/clavulanic acid
    • ampicillin/sulbactam

    Other infections
    • fluoroquinolones
    • cephalosporines (second and third generation)

    • Alternative
    • aminoglycosides
    • imipenem/Cilastin
    • aztreonam
    • colistin

    In ESBLs avoid cephalosporins. Carbapenemase producers (KPCs) are resistant to carbapenems, penicillins, cephalosporins, fluoroquinolones and aminoglycosides.

    List of antibiotics (Wikipedia)


Klebsiella pneumoniae colonies on agar cultivation media

Klebsiella pneumoniae on blood agar

klebsiella pneumoniae mucoid colonies on blood agar klebsiella pneumoniae in Petri dish on blood agar with non-hemolytic colonies characteristic growth of klebsiella on agar, non-hemolytic, mucoid colonies colonies of klebsiella on blood agar, gamma-hemolysis mucous klebsiella pneumoniae on blood agar klebsiella pneumoniae colony detail, gamma-hemolysis, gamma-hemolytic colonies colony appearance of klebsiella pneumoniae, microscopic appearance colonies of klebsiella on blood agar klebsiella pneumoniae colonies on blood agar k.pneumoniae on blood agar blood agar with non-hemolytic colonies of klebsiella pneumoniae hands with gloves holding Petri dishes with klebsiella pneumoniae bacteria on blood agar work wit klebsiella pneumoniae culture in biohazard box (flow-box) typical appearance and colony morphology of klebsiella pneumoniae on blood agar klebsiella pneumoniae mucoid colonies and consistence

Klebsiella pneumoniae on MacConkey

klebsiella pneumoniae macconkey plate, Petri dish klebsiella pneumoniae mucous colonies on MacConkey pure culture of klebsiella pneumoniae in Petri dish with MacConkey agar k.pneumoniae and p.aeruginosa macconkey plate, Petri dish klebsiella pneumoniae colonies in detail on MacConkey agar isolated lactose-positive colonies of Klebsiella on MacConkey klebsiella colonies on MacConkey lactose-positive klebsiella pneumoniae on MacConkey klebsiella pneumoniae growing on MacConkey klebsiella colony appearance on MacConkey klebsiella pneumoniae detail of colonies on mcconkey agar a Petri dish culture plate that demonstrated growth of Klebsiella pneumoniae

Klebsiella pneumoniae on Endo agar

klebsiella on Endo agar plate klebsiella pneumoniae colonies on Endo agar plate klebsiella pneumoniae and e.coli colony on Endo agar in detail klebsiella pneumoniae on Endo plate klebsiella pneumoniae and e.coli on Endo colony appearance of klebsiella pneumoniae on Endo klebsiella pneumoniae growth characteristics on Endo klebsiella pneumoniae growth characteristics on Endo and MacConkey lactose positive colonies of klebsiella pneumoniae on Endo lactose positive and negative colonies on Endo agar

Klebsiella pneumoniae on CLED agar

klebsiella pneumoniae growth characteristics on CLED agar klebsiella pneumoniae, e.coli, enterococcus faecalis on CLED

Other solid cultivation media for microbiology

klebsiella pneumoniae in Petri dish with tryptic soy agar klebsiella pneumoniae colonies appearance on tryptic soy agar klebsiella pneumoniae and enterococcus esculin hydrolysis

Klebsiella pneumoniae sensitivity to antibiotics

klebsiella antibiotic susceptibility klebsiella pneumoniae antibiotic resistant, ESBL positive klebsiella pneumoniae ESBL evidence carbapenem resistant bacteria on agar in Petri dish

Klebsiella pneumoniae identification

klebsiella pneumoniae basic tests for identification klebsiella pneumoniae biochemistry klebsiella pneumoniae urea positive, gas from glucose klebsiella pneumoniae colony morphology on agar media klebsiella pneumoniae tests for identification

Klebsiella pneumoniae Gram-stain

klebsiella pneumoniae Gram staining technique klebsiella pneumoniae Gram

Klebsiella pneumoniae SEM

klebsiella pneumoniae SEM micrograph klebsiella pneumoniae SEM microscopy

Useful Links

WIKIPEDIA
CENTERS FOR DISEASE CONTROL AND PREVENTION (CDC)