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Preliminary biochemical identification of non-fastidious Gram-negative bacteria

isolation plate

Bacterial colonies on the red slope(glucose fermentation) are lactose negative, non-gas producing from glucose, mannitol positive is Shigella sonnei. To confirm this identification you have to test other biochemical properties of the strain (usually a commercial test) and aglutination in shigella specific polyvalent and monovalent antisera.

Bacterial colonies on the blue slope(urea positive),hydrogen sulfide positive(black precipitate under cover-slip) is Budvicia aquatica. This bacterium is rare in clinical samples and most probably not nonpathoenic.

A FEW OTHER EXAMPLES OF BIOCHEMICAL PROPERTIES OF VARIOUS BACTERIAL STRAINS

isolation plate